IL-1 beta Rekombinanter Antikörper

IL-1 beta Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241364D5

Kat-Nr. : 84136-5-PBS

Synonyme

IL 1beta, Il1b, 241364D5, IL 1 beta, Il 1b

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  WB analysis of THP-1 using 84136-5-RR (same clone as 84136-5-PBS)

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 84136-5-RR (IL-1 beta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84136-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 84136-5-RR (same clone as 84136-5-PBS)

  LPS and Brefeldin A treated RAW264.7 cells were subjected to SDS PAGE followed by western blot with 84136-5-RR (IL-1 beta antibody) at dilution of 1:4000, incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84136-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84136-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84136-5-RR against Mouse IL-1 beta were performed. The affinity constant is 13.2 pM.

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Geprüfte Anwendungen

Produktinformation

84136-5-PBS bindet in WB, Indirect ELISA IL-1 beta und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-1 beta fusion protein Eg1577
• Vollständiger Name: interleukin 1 beta
• Berechnetes Molekulargewicht: 31kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: NM_008361.4
• Gene symbol: IL-1 beta
• Gene ID (NCBI): 16176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.