IL-10 Polyklonaler Antikörper

IL-10 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (5)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(117)

Kat-Nr. : 20850-1-AP

Synonyme

IL-10,IL10, CSIF, IL 10, IL10, IL10A



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRecombinant protein, ag14644

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20850-1-AP bindet in WB, IHC, IF, ELISA IL-10 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Affe, Hausschwein, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IL-10 fusion protein Ag14644
Vollständiger Name interleukin 10
Berechnetes Molekulargewicht 21 kDa
Beobachtetes Molekulargewicht20 kDa
GenBank-ZugangsnummerBC104252
Gene symbol IL-10
Gene ID (NCBI) 3586
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-10 antibody 20850-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Adv Sci (Weinh)

3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure

Authors - Jiabin Zhang
humanIF

Nat Commun

B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.

Authors - Johannes Griss
mouseIHC

Microbiome

Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice.

Authors - Hong Zheng
ratIHC

Acta Biomater

A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.

Authors - Yingzheng Zhao
ratIHC

Acta Biomater

Bio-inspired Glycosylated Nano-hydroxyapatites Enhance Endogenous Bone Regeneration by Modulating Macrophage M2 Polarization

Authors - Dan Qiao
mouseWB

Alzheimers Res Ther

Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis

Authors - Deng-Pan Wu