IL-10 Monoklonaler Antikörper

IL-10 Monoklonal Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

7B8G3

Kat-Nr. : 69018-2-PBS

Synonyme

NeutraKine® IL-10, IL-10,IL10, 7B8G3, CSIF, IL 10

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  Sandwich ELISA standard curve of MP50035-1

  Sandwich ELISA standard curve of MP50035-1, IL-10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69018-1-PBS. Detection antibody: 69018-2-PBS. Standard: HZ-1145. Range: 15.6-1000 pg/mL.

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  Sample test of MP50035-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 day or 5 days. For the PBMC supernatant cultured for 1 day, the mean IL-10 concentration was determined to be 431.4 pg/mL in unstimulated PBMC supernatant, 1,534.2 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean IL-10 concentration was determined to be 225.9 pg/mL in unstimulated PBMC supernatant, 634.8 pg/mL in PHA stimulated PBMC supernatant.

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  Affinity and Kinetic Characterization of 69018-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 69018-2-Ig against Human IL-10 were performed. The affinity constant is 0.426 nM.

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Produktinformation

69018-2-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test IL-10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: human IL-10 protein HZ-1145
• Vollständiger Name: interleukin 10
• Gene symbol: IL-10
• Gene ID (NCBI): 3586
• Konjugation: Unkonjugiert
• Form: Lyophilized Powder
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Wiederherstellung: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.