IL-10 Rekombinanter Antikörper

IL-10 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

240430G12

Kat-Nr. : 82191-3-RR

Synonyme

Il10, IL-10,IL10, 240430G12, CSIF, Il 10

---

• 

  WB analysis using 82191-3-RR

  Untreated, PMA, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82191-3-RR (Il10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 4 publications below

Produktinformation

82191-3-RR bindet in WB, IHC, ELISA IL-10 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-10 fusion protein Eg0026
• Vollständiger Name: interleukin 10
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: NM_010548
• Gene symbol: Il10
• Gene ID (NCBI): 16153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Publikationen

Species	Application	Title
mouse	IHC	Bioeng Transl Med; Macrophages overexpressing interleukin-10 target and prevent atherosclerosis: Regression of plaque formation and reduction in necrotic core; Authors - Mingyi Wang
mouse	WB	Front Immunol; Acute hypoxia modulate macrophage phenotype accompanied with transcriptome re-programming and metabolic re-modeling; Authors - Binda Sun
mouse	IHC	Inflamm Res; Luteolin modulates liver macrophage subtype polarization and play protective role in sepsis induced acute hepatic injury; Authors - Liangyong Deng
mouse	WB	Apoptosis; Itaconate promotes mitophagy to inhibit neuronal ferroptosis after subarachnoid hemorrhage; Authors - Guijun Wang
rat	IHC	Adv Healthc Mater; Extracellular Vesicles from Adipose-Derived Mesenchymal Stem Cells Combined with PEG Hydrogel Alleviate Maternal Simulated Birth Injury in a Rat Model; Authors - Qing Wang
rat	IHC	J Transl Med; Fluorinated hydroxyapatite conditions a favorable osteo-immune microenvironment via triggering metabolic shift from glycolysis to oxidative phosphorylation; Authors - Kaidi Chen