IL-11 Monoklonaler Antikörper

IL-11 Monoklonal Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F3A12

Kat-Nr. : 68135-1-PBS

Synonyme

IL11, 1F3A12, Adipogenesis inhibitory factor, AGIF, IL 11

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  WB analysis using 68135-1-Ig (same clone as 68135-1-PBS)

  Saos-2 cell supernatant (cells stimulated with 500ng/mL HGF for 4 days) were subjected to SDS PAGE followed by western blot with 68135-1-Ig (IL-11 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68135-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP50054-1

  Serum of sixteen individual healthy human donors was measured. All samples measured less than the lowest human IL-11 standard, 6.25 pg/mL.

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  Sandwich ELISA sample test of MP50054-1

  A549 cells were cultured unstimulated or stimulated with 2 ng/mL human TGF-β for 2 days. The mean IL-11 concentration was determined to be 2,309.9 pg/mL in unstimulated A549 supernatant, 94,433.7 pg/mL in stimulated A549 supernatant.

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  Sandwich ELISA standard curve of MP50054-1

  Sandwich ELISA standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair - PBS only. 68135-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag11199. 68135-1-PBS was biotinylated as the detection antibody. Range: 6.25-400 pg/mL.

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  Cytometric bead array sample test of MP50054-1

  Cytometric bead array of Saos-2 cells supernatant using MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Saos-2 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 3 days in normal culture media or HGF (Cat.NO. HZ-1084, 500 ng/mL) containing media. 50 μL of the supernatant from each day was analyzed using MP50054-1 (IL11 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time. Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

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  Cytometric bead array standard curve of MP50054-1

  Cytometric bead array standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Standard:Ag11199. Range: 1.563-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68135-1-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA IL-11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-11 fusion protein Ag11199
• Vollständiger Name: interleukin 11
• Berechnetes Molekulargewicht: 199 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC012506
• Gene symbol: IL-11
• Gene ID (NCBI): 3589
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.