IL-12A/IL-12 p35 Monoklonaler Antikörper

IL-12A/IL-12 p35 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

1E12H6

Kat-Nr. : 65277-1-PBS

Synonyme

IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35

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  FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite ® Plus 750 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite ® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unstimulated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65277-1-PBS bindet in FC (Intra) IL-12A/IL-12 p35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-12A/IL-12 p35 fusion protein HZ-1256
• Vollständiger Name: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC104982
• Gene symbol: IL-12A
• Gene ID (NCBI): 3592
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: -20°C. Aliquotieren ist bei -20^oC Lagerung nicht notwendig.

Hintergrundinformationen

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).