IL-12 p70 Rekombinanter Antikörper

IL-12 p70 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230295F4

Kat-Nr. : 83094-1-RR

Synonyme

IL-12, Interleukin 12, IL12, IL 12

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  WB analysis using 83094-1-RR

  Recombinant protein IL-12 p70(Eg0575) was subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83094-1-RR

  Recombinant protein IL-12B p40(Eg0431) were subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83094-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83094-1-RR against Human IL-12 p70 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83094-1-RR bindet in WB, ELISA IL-12 p70 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: IL-12 p70
• Beobachtetes Molekulargewicht: 70 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells.