IL-12A/IL-12 p35 Monoklonaler Antikörper

IL-12A/IL-12 p35 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E12H6

Kat-Nr. : CL647-65277

Synonyme

IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35

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  FC experiment of human PBMCs using CL647-65277

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65277

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): LPS and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65277 bindet in FC (Intra) IL-12A/IL-12 p35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-12A/IL-12 p35 fusion protein HZ-1256
• Vollständiger Name: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC104982
• Gene symbol: IL-12A
• Gene ID (NCBI): 3592
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).