IL-12/IL-23 p40 Rekombinanter Antikörper

IL-12/IL-23 p40 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241600F8

Kat-Nr. : 98277-1-PBS

Synonyme

IL12B, 241600F8, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40

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  FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

  1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98277-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

  1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL12B Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were stained with 0.25 ug Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98277-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98277-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98277-1-RR against Human IL-12/IL-23 p40 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98277-1-PBS bindet in FC (Intra) IL-12/IL-23 p40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• Berechnetes Molekulargewicht: 328 aa, 37 kDa
• GenBank-Zugangsnummer: BC067498
• Gene symbol: IL12B
• Gene ID (NCBI): 3593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).