IL-12 p70 Rekombinanter Antikörper

IL-12 p70 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230037B7

Kat-Nr. : 82878-2-RR

Synonyme

Il 12b, Il 12p40, IL-12 P35, IL-12 p70, Il12b, Il12p40, interleukin 12b, p40

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  WB analysis of Recombinant protein using 82878-2-RR

  Recombinant protein Eg0742 (IL-12 p70) were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82878-2-RR

  Recombinant protein Eg0742 (IL-12 p70) and Recombinant protein Eg1821 (IL-12A p35)were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82878-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82878-2-RR against Mouse IL-12 p70 were performed. The affinity constant is 7.37 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82878-2-RR bindet in WB, ELISA IL-12 p70 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: IL-12 p70
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.