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IL-12A/IL-12 p35 Rekombinanter Antikörper

IL-12A/IL-12 p35 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242887G5

Kat-Nr. : 85754-4-RR

Synonyme

IL-12, CLMF, CLMF p35, IL 12 subunit p35, IL 12A

Filter:
  • Western Blot (WB) analysis of various lysates using IL-12A/IL-12 p35 Recombinant antibody (85754-4-RR)

    WB analysis using 85754-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85754-4-RR (IL-12A/IL-12 p35 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IL-12A/IL-12 p35 Recombinant monoclonal antibody (85754-4-RR)

    IF Staining of THP-1 using 85754-4-RR

    Immunofluorescent analysis of (4% PFA) fixed IFN gamma and LPS treated THP-1 cells using IL-12A/IL-12 p35 antibody (85754-4-RR, Clone: 242887G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRecombinant protein
Erfolgreiche Detektion in IF/ICCIFN gamma and LPS treated THP-1 cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

85754-4-RR bindet in WB, IF/ICC, ELISA IL-12A/IL-12 p35 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IL-12A/IL-12 p35 fusion protein Eg3341
Vollständiger Name interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht65 kDa
GenBank-ZugangsnummerBC104982
Gene symbol IL-12A
Gene ID (NCBI) 3592
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-12A (Interleukin-12A) is a subunit of the cytokine IL-12, which is also known as IL-12 p35. IL-12 is a heterodimeric cytokine composed of two subunits: IL-12A (p35) and IL-12B (p40). This cytokine plays a crucial role in the immune response, particularly in the activation and differentiation of T cells and natural killer (NK) cells. This antibody recognizes IL-12A and IL-12 P70, which consists of IL-12A and B, but does not recognize IL-12B.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-12A/IL-12 p35 antibody 85754-4-RRProtokoll herunterladen
IF protocol for IL-12A/IL-12 p35 antibody 85754-4-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen