IL-12RB1/CD212 Rekombinanter Antikörper

IL-12RB1/CD212 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241661H1

Kat-Nr. : 98149-1-RR

Synonyme

IL12RB1, IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1

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  FC experiment of human PBMCs using 98149-1-RR

  1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human IL-12RB1/CD212 Rabbit Recombinant Antibody (98149-1-RR, Clone: 241661H1) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human IL-12RB1/CD212 Rabbit Recombinant Antibody (98149-1-RR, Clone: 241661H1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98149-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98149-1-RR against Human IL-12RB1/CD212 were performed. The affinity constant is 2.2 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: anti-CD3/CD28 treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98149-1-RR bindet in FC IL-12RB1/CD212 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 12 receptor, beta 1
• Berechnetes Molekulargewicht: 662 aa, 73 kDa
• GenBank-Zugangsnummer: BC029121
• Gene symbol: IL12RB1
• Gene ID (NCBI): 3594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-12 receptor subunit beta-1 (IL-12RB1), also named as CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. IL-12RB1 localizes in the membrane and functions as an interleukin receptor which binds IL-12 with low affinity and is involved in IL-12 transduction. Associated with IL-12RB2 it forms a functional, high affinity receptor for IL-12. It can also associate with IL23R to form the interleukin-23 receptor which functions in IL-23 signal transduction probably through activation of the Jak-Stat signaling cascade.