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IL-13 Rekombinanter Antikörper

IL-13 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240771B9

Kat-Nr. : 83935-1-PBS

Synonyme

Il13, interleukin 13, 240771B9

Filter:
  • Western Blot (WB) analysis of EL-4 cells using IL-13 Recombinant antibody (83935-1-RR)

    WB analysis of EL-4 using 83935-1-RR (same clone as 83935-1-PBS)

    Untreated EL-4 and EL-4 (treated with 50ng/mL PMA and Ionomycin 500ng/mL for 6h, replaced the culture medium with Brefeldin A for 18 hours) cells were subjected to SDS PAGE followed by western blot with 83935-1-RR (IL-13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83935-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83935-1-RR

    Affinity and Kinetic Characterization of 83935-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83935-1-RR against Rat IL-13 were performed. The affinity constant is 0.419 nM.

Geprüfte Anwendungen

Produktinformation

83935-1-PBS bindet in WB, ELISA IL-13 und zeigt Reaktivität mit Maus, Ratten

Getestete Reaktivität Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name interleukin 13
Berechnetes Molekulargewicht14kDa
Beobachtetes Molekulargewicht12 kDa
GenBank-ZugangsnummerNM_053828.1
Gene symbol Il13
Gene ID (NCBI) 116553
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rat IL-13 is a kind of cytokines, which plays important roles in allergic inflammation and immune response to parasite infection. (PubMed:9366558). Rat IL-13 displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (PubMed:9366558).