IL-13 Rekombinanter Antikörper

IL-13 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240771B9

Kat-Nr. : 83935-1-RR

Synonyme

Il13, interleukin 13, 240771B9

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  WB analysis of EL-4 using 83935-1-RR

  Untreated EL-4 and EL-4 (treated with 50ng/mL PMA and Ionomycin 500ng/mL for 6h, replaced the culture medium with Brefeldin A for 18 hours) cells were subjected to SDS PAGE followed by western blot with 83935-1-RR (IL-13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83935-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83935-1-RR against Rat IL-13 were performed. The affinity constant is 0.419 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, Ionomycin and Brefeldin A treated EL-4 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83935-1-RR bindet in WB, ELISA IL-13 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: interleukin 13
• Berechnetes Molekulargewicht: 14kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: NM_053828.1
• Gene symbol: Il13
• Gene ID (NCBI): 116553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rat IL-13 is a kind of cytokines, which plays important roles in allergic inflammation and immune response to parasite infection. (PubMed:9366558). Rat IL-13 displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (PubMed:9366558).