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IL-13Ra1/CD213a1 Polyklonaler Antikörper

IL-13Ra1/CD213a1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27916-1-AP

Synonyme

IL13RA1, Cancer/testis antigen 19, CD213A1, CT19, IL 13 receptor subunit alpha 1

Filter:
  • Western Blot (WB) analysis of various lysates using IL-13Rα1 Polyclonal antibody (27916-1-AP)

    WB analysis using 27916-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27916-1-AP (IL-13RA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHT-29-Zellen, Jurkat-Zellen, Maus-Skelettmuskelgewebe, Raji-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27916-1-AP bindet in WB, ELISA IL-13Ra1/CD213a1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IL-13Ra1/CD213a1 fusion protein Ag25542
Vollständiger Name interleukin 13 receptor, alpha 1
Berechnetes Molekulargewicht 49 kDa
Beobachtetes Molekulargewicht70 kDa
GenBank-ZugangsnummerBC009960
Gene symbol IL-13Rα1
Gene ID (NCBI) 3597
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a 65- to 70-kd glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-13Ra1/CD213a1 antibody 27916-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Ciara (Verified Customer) (01-30-2024)

Positive staining in N2a cells

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Neuro-2a mouse neuroblastoma cell line