IL-15 Rekombinanter Antikörper

IL-15 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

230285B3

Kat-Nr. : 98036-1-RR

Synonyme

230285B3, AI503618, Il15, interleukin 15

---

• 

  FC experiment of Mouse PBMCs using 98036-1-RR

  1x10^6 Mouse PBMCs were intracellularly stained with 0.25 ug Anti-Mouse IL-15 Rabbit Recombinant Antibody (98036-1-RR, Clone: 230285B3) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

• 

  Affinity and Kinetic Characterization of 98036-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98036-1-RR against Mouse IL-15 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Mouse PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98036-1-RR bindet in FC (Intra) IL-15 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-15 fusion protein Eg0359
• Vollständiger Name: interleukin 15
• Berechnetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: NM_001254747
• Gene symbol: IL-15
• Gene ID (NCBI): 16168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

IL-15 is a 4-α-helix bundle cytokine playing a pivotal role in stimulation of both innate and adaptive immune cells. It is produced primarily by keratinocytes, skeletal muscle cells, monocytes, and CD4+ T cells. It is a member of the common gamma chain family. The members of the common gamma chain family include the IL-2, IL-4, IL-7, IL-9, and IL-21 and require binding to the common gamma chain receptor for activation. It can be used in growth and maintenance of T and NK cells. It is also shown to be used in proliferation and functional effect of T cells for adoptive cell therapy. It is a glycosylated protein and HumanKine IL-15 appear as 12.5-25 kDa bands (PMID: 24587813, 26627006, 27849617, 31250350)