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IL-15RA Monoklonaler Antikörper

IL-15RA Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5E3E1

Kat-Nr. : 60138-1-PBS

Synonyme

IL15RA, 5E3E1, CD215, IL 15 receptor subunit alpha, IL 15R alpha

Filter:
  • Western Blot (WB) analysis of Raji cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of Raji using 60138-1-Ig (same clone as 60138-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of Daudi using 60138-1-Ig (same clone as 60138-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of HeLa using 60138-1-Ig (same clone as 60138-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60138-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

60138-1-PBS bindet in WB, Indirect ELISA IL-15RA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen IL-15RA fusion protein Ag10200
Vollständiger Name interleukin 15 receptor, alpha
Berechnetes Molekulargewicht 231 aa, 24 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC107777
Gene symbol IL-15RA
Gene ID (NCBI) 3601
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).