IL-15RA Rekombinanter Antikörper

IL-15RA Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

242689E1

Kat-Nr. : PE-98380-3

Synonyme

IL15RA, CD215, IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA

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  FC experiment of human PBMCs using PE-98380-3

  1x10^6 untreated or LPS and IFN-γ treated human PBMCs were surface stained with APC Anti-Human CD14 and 5 ul PE Anti-Human IL-15RA Rabbit RecAb (PE-98380-3, Clone:242689E1). Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-98380-3

  1x10^6 LPS and IFN-γ treated human PBMCs were surface stained with 5 ul PE Anti-Human IL15RA Rabbit RecAb (PE-98380-3, Clone: 242689E1) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human IL15RA Rabbit RecAb (PE-98380-3, Clone: 242689E1) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS and IFN-γ treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-98380-3 bindet in FC IL-15RA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 15 receptor, alpha
• Berechnetes Molekulargewicht: 231 aa, 24 kDa
• GenBank-Zugangsnummer: BC107777
• Gene symbol: IL-15RA
• Gene ID (NCBI): 3601
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897).