IL-17A Monoklonaler Antikörper

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• KD/KO Validated

IL-17A Monoklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3D5

Kat-Nr. : 66148-1-PBS

Synonyme

IL 17, IL17, IL-17, IL17A, 1B3D5

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  WB analysis using 66148-1-Ig (same clone as 66148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66148-1-Ig (same clone as 66148-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66148-1-Ig (same clone as 66148-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66148-1-Ig (same clone as 66148-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66148-1-PBS bindet in WB, IF-P, ELISA IL-17A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-17A fusion protein Ag3733
• Vollständiger Name: interleukin 17A
• Berechnetes Molekulargewicht: 155 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC067505
• Gene symbol: IL-17A
• Gene ID (NCBI): 3605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.