IL-17A Monoklonaler Antikörper

IL-17A Monoklonal Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

1E3H1

Kat-Nr. : 69021-2-PBS

Synonyme

NeutraKine® IL-17A, IL 17, IL17, IL-17, IL17A

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  Sandwich ELISA standard curve of MP50004-1

  Sandwich ELISA standard curve of MP50004-1, IL17A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69021-2-PBS. Detection antibody: 69021-1-PBS. Standard: HZ-1113. Range: 31.25-2000 pg/mL.

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  Sample test of MP50004-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. The mean IL17A concentration was undetected in unstimulated PBMC supernatant, 126.6 pg/mL in PBMC supernatant stimulated with PHA for 1 day and 251.8 pg/mL in PBMC supernatant stimulated with PHA for 5 days.

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  Sample test of MP50004-1

  Twenty-four serum samples from volunteers were evaluated for human IL-17A in this assay. twenty-two samples measured less than the lowest standard, 31.25 pg/mL. Two samples measured 98.5 pg/mL and 107.5 pg/mL respectively. No medical histories were available for the donors used in this study.

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  Affinity and Kinetic Characterization of 69021-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 69021-2-Ig against Human IL-17A were performed. The affinity constant is 0.29 nM.

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Produktinformation

69021-2-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test IL-17A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: human IL-17A protein HZ-1113
• Vollständiger Name: interleukin 17A
• Gene symbol: IL-17A
• Gene ID (NCBI): 3605
• Konjugation: Unkonjugiert
• Form: Lyophilized Powder
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Endotoxin: <0.1 EU/μg
• Wiederherstellung: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.