IL-17A Rekombinanter Antikörper

IL-17A Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230038B1

Kat-Nr. : 82905-1-RR

Synonyme

Il17a, Interleukin17A, interleukin 17A, Il17, IL 17A

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  WB analysis of EL-4 using 82905-1-RR

  PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 82905-1-RR (IL-17A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82905-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82905-1-RR against Mouse IL-17A were performed. The affinity constant is 7.59 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, Ionomycin and Brefeldin A treated EL-4 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82905-1-RR bindet in WB, ELISA IL-17A und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 17A
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 14 kDa, 17 kDa
• GenBank-Zugangsnummer: NM-010552
• Gene symbol: Il17a
• Gene ID (NCBI): 16171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.