IL-17A Rekombinanter Antikörper

IL-17A Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250413G7

Kat-Nr. : 98564-1-PBS

Synonyme

Il17a, Interleukin 17A

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  FC experiment of Transfected HEK-293T using 98564-1-RR (same clone as 98564-1-PBS)

  1x10^6 HEK-293T cells (left) and IL-17A transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-17A Rabbit RecAb (98564-1-RR, Clone: 250413G7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98564-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98564-1-RR (same clone as 98564-1-PBS)

  1x10^6 HEK-293Tcells (left) and IL-17A transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-17A Rabbit RecAb (98564-1-RR, Clone: 250413G7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98564-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98564-1-PBS bindet in FC IL-17A und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-17A fusion protein Eg3339
• Vollständiger Name: similar to Interleukin-17 precursor (IL-17) (Cytotoxic T lymphocyte-associated antigen 8) (CTLA-8)
• Berechnetes Molekulargewicht: 17kDa
• Gene symbol: LOC301289
• Gene ID (NCBI): 301289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.