IL-17F Monoklonaler Antikörper

IL-17F Monoklonal Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

1C5C6

Kat-Nr. : 68666-2-PBS

Synonyme

IL17F, Cytokine ML 1, Cytokine ML-1, IL 17F, interleukin 17F

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  Sandwich ELISA standard curve of MP50006-1

  Sandwich ELISA standard curve of MP50006-1, IL-17F Monoclonal Matched Antibody Pair, PBS Only. 68666-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1116. 68666-2-PBS was HRP conjugated as the detection antibody Range: 62.5-4000 pg/mL.

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  Sample test of MP50006-1

  Serum of sixteen individual healthy human donors was measured. Fourteen measured less than the lowest human IL-17F standard, 62.5 pg/mL. Two samples read 46.2 pg/mL and 277.7 pg/mL.

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  Sample test of MP50006-1

  Human peripheral blood mononuclear cells (PBMC) was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were unstimulated or stimulated with 1 μg/mL CD3 for 4 days. An aliquot of the cell culture supernatant was removed, the mean IL-17F concentration was undetectable in unstimulated PBMC supernatant, 3,282.8 pg/mL in CD3 stimulated PBMC supernatant.

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Geprüfte Anwendungen

Produktinformation

68666-2-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test IL-17F und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-17F fusion protein HZ-1116
• Vollständiger Name: interleukin 17F
• Gene symbol: IL-17F
• Gene ID (NCBI): 112744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.