IL-18R1/CD218a Rekombinanter Antikörper

IL-18R1/CD218a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241673D3

Kat-Nr. : 98150-2-RR

Synonyme

IL18R1, 241673D3, CD218 antigen-like family member A, CD218a, EC:3.2.2.6

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  FC experiment of human PBMCs using 98150-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-18R1/CD218a Rabbit Recombinant Antibody (98150-2-RR, Clone:241673D3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98150-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-18R1/CD218a Rabbit Recombinant Antibody (98150-2-RR, Clone: 241673D3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9)(blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98150-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98150-2-RR against Human IL-18R1/CD218a were performed. The affinity constant is 0.915 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood leukocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98150-2-RR bindet in FC IL-18R1/CD218a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 18 receptor 1
• Berechnetes Molekulargewicht: 541 aa, 62 kDa
• GenBank-Zugangsnummer: BC093975
• Gene symbol: IL18R1
• Gene ID (NCBI): 8809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

IL-18R1 (CD218a) is a component of the interleukin-18 receptor (IL-18R) complex, which is critical for the signaling of IL-18, a pro-inflammatory cytokine. This receptor complex, consisting of IL-18R1 and the IL-18 receptor accessory protein (IL-18RAP), belongs to the immunoglobulin-like family of interleukin-1 receptors (PMID: 35461924). IL-18R1 plays a significant role in inducing the production of interferon-gamma (IFN-γ) and other inflammatory cytokines, contributing to innate and adaptive immune responses (PMID: 2550053). It is also suggested to play a role in hypertension and chronic kidney disease, where the IL-18/IL-18R1 signaling axis may have diagnostic and therapeutic potential (PMID: 35461924).