IL-1R1 Rekombinanter Antikörper

IL-1R1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241392G7

Kat-Nr. : 84094-6-RR

Synonyme

IL-1R-1, Il1r1, EC:3.2.2.6, CD121a, CD121 antigen-like family member A

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  WB analysis of mouse liver using 84094-6-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 84094-6-RR (IL-1R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of EL-4-B5 using 84094-6-RR

  EL-4-B5 cells were subjected to SDS PAGE followed by western blot with 84094-6-RR (IL-1R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84094-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84094-6-RR (IL-1R1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84094-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84094-6-RR (IL-1R1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 84094-6-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using IL-1R1 antibody (84094-6-RR, Clone: 241392G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84094-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84094-6-RR against Mouse IL-1R1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, EL-4-B5 cells
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84094-6-RR bindet in WB, IHC, IF/ICC, ELISA IL-1R1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: interleukin 1 receptor, type I
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 75-100 kDa
• GenBank-Zugangsnummer: NM_008362.2
• Gene symbol: Il1r1
• Gene ID (NCBI): 16177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-1R1 (IL-1 receptor type 1, also known as p80, IL-1R-alpha or CD121a) is a 80 kDa transmembrane glycoprotein and a member of the interleukin-1 receptor family (PMID: 35163653; 29248002). It is a receptor for IL-1α, IL-1β, and interleukin-1 receptor antagonist (IL-1Ra). IL-1α and IL-1β interact with the extracellular domain of IL-1R1, triggering the recruitment of an accessory receptor, the IL-1RAcP, resulting in a functional receptor complex that initiates IL-1R1 signaling cascades (PMID: 35163653). IL-1Ra competes with active IL-1 and blocks binding to their common activating receptor IL-1R1 and, thus, inhibits IL-1 signaling (PMID: 35332327; 11641511). It has been reported that IL-1R1 may also bind IL-38 (PMID: 29248002). IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be released from the cell surface following proteolytic cleavage, generating a soluble form of 55-60 kDa (PMID: 17324958).