IL-1RA/IL-1RN Rekombinanter Antikörper

IL-1RA/IL-1RN Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242317D2

Kat-Nr. : 85672-3-PBS

Synonyme

IL1 inhibitor, IL1RA, IL-1ra, Il1rn, IL-1RN

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  WB analysis using 85672-3-RR (same clone as 85672-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85672-3-RR (IL-1RA/IL-1RN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85672-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 85672-3-RR (same clone as 85672-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 85672-3-RR (IL-1RA/IL-1RN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85672-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 85672-3-RR (same clone as 85672-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 85672-3-RR (IL-1RA/IL-1RN antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85672-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02044-2

  Cytometric bead array standard curve of MP02044-2, RAT IL-1RA  Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85672-3-PBS. Detection antibody: 85672-2-PBS. Standard: Eg3206. Range: 1.563-200 ng/mL.

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  Cytometric bead array standard curve of MP02044-1

  Cytometric bead array standard curve of MP02044-1, RAT IL-1RA  Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85672-1-PBS. Detection antibody: 85672-3-PBS. Standard: Eg3206. Range: 1.563-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

85672-3-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA IL-1RA/IL-1RN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-1RA/IL-1RN fusion protein Eg3206
• Vollständiger Name: interleukin 1 receptor antagonist
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: NM_022194.2
• Gene symbol: Il1rn
• Gene ID (NCBI): 60582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.