IL-1RA Rekombinanter Antikörper

IL-1RA  Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240208G5

Kat-Nr. : 83335-4-PBS

Synonyme

Il1rn, IL-1RN, 240208G5, IL 1ra, IL-1RA

---

• 

  WB analysis of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 83335-4-RR (IL-1RN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00353-2

  Cytometric bead array standard curve of MP00353-2, MOUSE IL-1RA Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83335-4-PBS. Detection antibody: 83335-3-PBS. Standard: Eg0685. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83335-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83335-4-RR against Mouse IL-1RN were performed. The affinity constant is 1.75 nM.

---

Geprüfte Anwendungen

Produktinformation

83335-4-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA IL-1RA  und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-1RA fusion protein Eg0685
• Vollständiger Name: interleukin 1 receptor antagonist
• Berechnetes Molekulargewicht: 18KD
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_031167.5
• Gene symbol: IL-1RA
• Gene ID (NCBI): 16181
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.