IL-2 Monoklonaler Antikörper

IL-2 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F11C5

Kat-Nr. : 60306-1-PBS

Synonyme

IL2, 2F11C5, IL 2, interleukin 2, Interleukin-2

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  WB analysis using 60306-1-Ig (same clone as 60306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60306-1-Ig (same clone as 60306-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60306-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA IL-2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-2 fusion protein Ag10199
• Vollständiger Name: interleukin 2
• Berechnetes Molekulargewicht: 153 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC070338
• Gene symbol: IL2
• Gene ID (NCBI): 3558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.