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IL-2 Rekombinanter Antikörper

IL-2 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240358B1

Kat-Nr. : 83547-3-PBS

Synonyme

Il2, 240358B1, Il 2, interleukin 2, Interleukin-2

Filter:
  • Western Blot (WB) analysis of EL-4 cells using IL-2 Recombinant antibody (83547-3-RR)

    WB analysis of EL-4 using 83547-3-RR (same clone as 83547-3-PBS)

    Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 83547-3-RR (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83547-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83547-3-RR

    Affinity and Kinetic Characterization of 83547-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83547-3-RR against Mouse IL-2 were performed. The affinity constant is 36.5 nM.

Geprüfte Anwendungen

Produktinformation

83547-3-PBS bindet in WB, Indirect ELISA IL-2 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IL-2 fusion protein Eg0762
Vollständiger Name interleukin 2
Berechnetes Molekulargewicht 19 kDa
Beobachtetes Molekulargewicht15 kDa
GenBank-Zugangsnummer NM_008366
Gene symbol IL-2
Gene ID (NCBI) 16183
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-2 (Il2) is a protein initially identified as a T cell growth factor. Il2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that Il2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (Il2R). Il2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.