IL-2 Rekombinanter Antikörper

IL-2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240358B1

Kat-Nr. : 83547-3-RR

Synonyme

Il2, 240358B1, Il 2, interleukin 2, Interleukin-2

---

• 

  WB analysis of EL-4 using 83547-3-RR

  Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 83547-3-RR (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83547-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83547-3-RR against Mouse IL-2 were performed. The affinity constant is 36.5 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, Ionomycin and Brefeldin A treated EL-4 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83547-3-RR bindet in WB, ELISA IL-2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-2 fusion protein Eg0762
• Vollständiger Name: interleukin 2
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: NM_008366
• Gene symbol: IL-2
• Gene ID (NCBI): 16183
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-2 (Il2) is a protein initially identified as a T cell growth factor. Il2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that Il2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (Il2R). Il2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.