• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NeutraKine® IL-12/IL-23 p40 Monoklonaler Antikörper

NeutraKine® IL-12/IL-23 p40 Monoklonal Antikörper für Neutralization, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

Neutralization, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A9H6

Kat-Nr. : 69006-1-Ig

Synonyme

Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), IL 23, IL-12 subunit p40, IL-12/IL-23 p40, IL12B, IL-12B, IL-23 p40, IL23A, IL-23B, IL23P19, NeutraKine® IL-23 p40, SGRF

Galerie der Validierungsdaten

Filter:
  • Neutralization experiment of NeutraKine® IL-23 p40 using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

    Recombinant human IL-23 (Cat.NO. HZ-1254) induces invitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL.

  • Neutralization experiment of NeutraKine® IL-23 p40 using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

    Recombinant human IL-23 (Cat.NO. HZ-1254) induces in vitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL. The NeutraControl mouse anti-human IL-23 p40 monoclonal antibody 69506-1-Ig could recognize human IL-23, but could not neutralize human IL-23 in the range of 0.244-4000ng/mL(green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    ELISA experiment of Recombinant protein using 69006-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-23 (Cat.NO. HZ-1254) at 70 ng/well followed by blocking and adding serial diluted IL-23 antibody 69006-1-Ig and 69506-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

  • ELISA experiment of Recombinant protein using NeutraKine® IL-12/IL-23 p40 Monoclonal antibody (69006-1-Ig)

    ELISA experiment of Recombinant protein using 69006-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-12 (Cat.NO. HZ-1256) and IL-12 p40 (Cat.NO. HZ-1321) respectively at 70 ng/well followed by blocking and adding serial diluted 69029-1-Ig, 69529-1-Ig and 69006-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggests that 69029-1-Ig and 69529-1-Ig only recognize IL-12(p35 and p40 heterodimer) but not recognize IL-12/IL-23 p40.

Neutralization

Produktinformation

69006-1-Ig bindet in Neutralization, ELISA NeutraKine® IL-12/IL-23 p40 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen human Humankine IL-12/IL-23 p40 protein HZ-1254
Vollständiger Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Berechnetes Molekulargewicht 328 aa, 37 kDa
GenBank-ZugangsnummerBC067498
Gene symbol IL12B
Gene ID (NCBI) 51561,3593
Konjugation Unkonjugiert
Form Lyophilized Powder
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer Sterile PBS.
Endotoxin<0.1 EU/μg
WiederherstellungThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Hintergrundinformationen

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

This antibody can be used to neutralize the bioactivity of IL-12/IL-23 p40.