IL-2RG/CD132 Rekombinanter Antikörper

IL-2RG/CD132 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242020H6

Kat-Nr. : 98234-1-RR

Synonyme

IL2RG, IL 2RG, IL 2R subunit gamma, IL 2 receptor subunit gamma, gammaC

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  FC experiment of human PBMCs using 98234-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR, Clone: 242020H6), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98234-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR, Clone: 242020H6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98234-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98234-1-RR against Human IL-2RG/CD132 were performed. The affinity constant is 1.55 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98234-1-RR bindet in FC IL-2RG/CD132 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 2 receptor, gamma (severe combined immunodeficiency)
• Berechnetes Molekulargewicht: NM_000206.3
• GenBank-Zugangsnummer: NM_000206.3
• Gene symbol: IL2RG
• Gene ID (NCBI): 3561
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL-2RA), beta (IL-2RB), and gamma (IL-2RG). IL-2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL-2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.