IL-3 Rekombinanter Antikörper

IL-3 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240417E1

Kat-Nr. : 98363-1-PBS

Synonyme

IL3, IL 3, Interleukin 3, Interleukin-3, Multipotential colony-stimulating factor

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  FC experiment of human PBMCs using 98363-1-RR (same clone as 98363-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Treated human PBMCs were pre-stimulated with anti-human CD3/CD28 and recombinant human IL-2 and IL-4. This data was developed using the same antibody clone with 98363-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98363-1-RR (same clone as 98363-1-PBS)

  1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Treated human PBMCs were pre-stimulated with anti-human CD3/CD28 and recombinant human IL-2 and IL-4. This data was developed using the same antibody clone with 98363-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98363-1-PBS bindet in FC (Intra) IL-3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-3 fusion protein Eg0829
• Vollständiger Name: interleukin 3 (colony-stimulating factor, multiple)
• Berechnetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: NM_000588.3
• Gene symbol: IL-3
• Gene ID (NCBI): 3562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. (PMID: 24103757;2698876;2809196)