IL-33 Monoklonaler Antikörper

IL-33 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A9C7

Kat-Nr. : 66235-1-PBS

Synonyme

C9orf26, DKFZp586H0523, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

---

• 

  WB analysis of human lung using 66235-1-Ig (same clone as 66235-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66235-1-Ig (same clone as 66235-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

• 

  WB analysis of hTERT-RPE1 using 66235-1-Ig (same clone as 66235-1-PBS)

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66235-1-Ig (same clone as 66235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66235-1-Ig (same clone as 66235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66235-1-PBS bindet in WB, ELISA IL-33 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-33 fusion protein Ag21430
• Vollständiger Name: interleukin 33
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC047085
• Gene symbol: IL-33
• Gene ID (NCBI): 90865
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.