IL-4 Monoklonaler Antikörper

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• KD/KO Validated

IL-4 Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1G2C5

Kat-Nr. : 66142-1-PBS

Synonyme

IL4, 1G2C5, B-cell stimulatory factor 1, BSF-1, IL 4

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  WB analysis of Jurkat using 66142-1-Ig (same clone as 66142-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66142-1-Ig (IL-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66142-1-Ig (same clone as 66142-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66142-1-Ig (same clone as 66142-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 66142-1-Ig (same clone as 66142-1-PBS)

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human IL-4 (66142-1-Ig, Clone:1G2C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66142-1-PBS bindet in WB, IHC, IF-P, FC (Intra), ELISA IL-4 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-4 fusion protein Ag9940
• Vollständiger Name: interleukin 4
• Berechnetes Molekulargewicht: 153 aa, 17 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC070123
• Gene symbol: IL-4
• Gene ID (NCBI): 3565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.