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IL-4 Rekombinanter Antikörper

IL-4 Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241249E11

Kat-Nr. : 84422-3-PBS

Synonyme

B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1, Il4

Filter:
  • Sandwich ELISA standard curve of MP01293-4

    Sandwich ELISA standard curve of MP01293-4

    Sandwich ELISA standard curve of MP01293-4, Rat IL-4 Recombinant Matched Antibody Pair - PBS only. 84422-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1113. 84422-5-PBS was Biotin conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01293-4

    Sample test of MP01293-4

    Serum of eight individual healthy human donors was measured. Seven measured less than the lowest rat IL-4 standard, 39.1 pg/mL. One sample read 18.7 pg/mL.

  • Sample test of MP01293-4

    Sample test of MP01293-4

    One rat thymus was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 5 μg/mL of PHA and 50 ng/mL of PMA for 7 days. An aliquot of the cell culture supernatant was removed, assayed for rat IL-4 and measured 98.5 pg/mL.

  • Affinity and Kinetic Characterization of 84422-3-PBS

    Affinity and Kinetic Characterization of 84422-3-PBS

    Biolayer interferometry (BLl) kinetic assays of 84422-3-PBS against Rat IL-4 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84422-3-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test IL-4 und zeigt Reaktivität mit Ratten

Getestete Reaktivität Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IL-4 fusion protein Eg1113
Vollständiger Name interleukin 4
Berechnetes Molekulargewicht16kDa
GenBank-ZugangsnummerNM_201270.1
Gene symbol IL-4
Gene ID (NCBI) 287287
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.