IL-4RA/CD124 Rekombinanter Antikörper

IL-4RA/CD124 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242262E3

Kat-Nr. : 84758-5-PBS

Synonyme

242262E3, CD124, IL-4 receptor subunit alpha, Il4r, IL-4R subunit alpha

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  WB analysis of NIH/3T3 using 84758-5-RR (same clone as 84758-5-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84758-5-RR (IL-4 R alpha/CD124 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84758-5-PBS bindet in WB, IHC, Indirect ELISA IL-4RA/CD124 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-4RA/CD124 fusion protein Eg2176
• Vollständiger Name: interleukin 4 receptor, alpha
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_001008700.3
• Gene symbol: Il4ra
• Gene ID (NCBI): 16190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.