IL-4RA/CD124 Monoklonaler Antikörper

IL-4RA/CD124 Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4C11

Kat-Nr. : CL488-67051

Synonyme

IL4R, 1E4C11, CD124, IL 4 binding protein, IL 4 receptor subunit alpha

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  IF Staining of human tonsillitis using CL488-67051

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite® Plus 488 IL-4RA/CD124 antibody (CL488-67051, Clone: 1E4C11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Daudi using CL488-67051

  Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CoraLite® Plus 488 IL-4RA/CD124 antibody (CL488-67051, Clone: 1E4C11 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in IF/ICC: Daudi-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67051 bindet in IF/ICC, IF-P IL-4RA/CD124 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-4RA/CD124 fusion protein Ag28411
• Vollständiger Name: interleukin 4 receptor
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_000418
• Gene symbol: IL-4R
• Gene ID (NCBI): 3566
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.