IL-5 Rekombinanter Antikörper

IL-5 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240915D12

Kat-Nr. : 98178-1-PBS

Synonyme

IL5, Interleukin-5, Interleukin 5, IL 5, B-cell differentiation factor I

---

• 

  FC experiment of human PBMCs using 98178-1-RR (same clone as 98178-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-5 Rabbit Recombinant Antibody (98178-1-RR, Clone: 240915D12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98178-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98178-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98178-1-RR against Human IL-5 were performed. The affinity constant is 0.689 nM.

---

Geprüfte Anwendungen

Produktinformation

98178-1-PBS bindet in FC (Intra) IL-5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 5 (colony-stimulating factor, eosinophil)
• Berechnetes Molekulargewicht: 134 aa, 15 kDa
• GenBank-Zugangsnummer: BC066282
• Gene symbol: IL5
• Gene ID (NCBI): 3567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity. (PMID: 21986312, 22174445, 19819937, 16895557)