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IL-6 Rekombinanter Antikörper

IL-6 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E9

Kat-Nr. : 81077-1-RR

Synonyme

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, Interferon beta 2, Interleukin 6

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HUVEC cells using IL-6 Recombinant antibody (81077-1-RR)

    WB analysis of HUVEC using 81077-1-RR

    Untreated HUVEC cells, HUVEC cells (treated with 1 µg/ml LPS for 24 hours, then add protein transport inhibitor last 16 hours) were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-87 MG cells using IL-6 Recombinant antibody (81077-1-RR)

    WB analysis of U-87 MG using 81077-1-RR

    Protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHUVEC-Zellen, Protein transport inhibitor treated U-87 MG cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

81077-1-RR bindet in WB, ELISA IL-6 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IL-6 fusion protein Ag10565
Vollständiger Name interleukin 6 (interferon, beta 2)
Berechnetes Molekulargewicht 212 aa, 24 kDa
Beobachtetes Molekulargewicht20 kDa
GenBank-ZugangsnummerBC015511
Gene symbol IL6
Gene ID (NCBI) 3569
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for IL-6 antibody 81077-1-RRProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen