IL-6 Rekombinanter Antikörper

IL-6 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240003G2

Kat-Nr. : 83179-2-PBS

Synonyme

IL6, Ifnb2, IL 6, ILg6, interleukin 6

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  Sandwich ELISA standard curve of MP00264-2

  Sandwich ELISA standard curve of MP00264-2, Rat IL-6 Monoclonal Matched Antibody Pair - PBS only. 83179-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0800. 83179-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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  Sample test of MP00264-2

  Three rats were given an intraperitoneal injection of 100 μg/kg of LPS. All ratswere killed 4 hours after receiving LPS. Spleens were harvested and broken into individual cells by using homogenizer. Ratspleen conditioned media wascollected after culturing in 60 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 1 μg/mL of LPS for 18 hours. An aliquot of the cell culture supernatant wasremoved, assayed for rat IL-6 and measured 6,449.6 pg/mL.

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  Sample test of MP00264-2

  One rat spleen was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant was removed, assayed for rat IL-6 and measured 179.7 pg/mL.

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  Sample test of MP00264-2

  Sixteen rat serum samples were evaluated for rat IL-6 in this assay. Fourteen samples were measured belowthe minimum standard of 62.5 pg/mL. The measured results of the two samples were 117.15 pg/mL and 133.51 pg/mL, respectively.

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  Cytometric bead array standard curve of MP00264-2

  Cytometric bead array standard curve of MP00264-2, Rat IL-6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83179-2-PBS. Detection antibody: 83179-3-PBS. Standard: Eg0800. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83179-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 83179-2-PBS against Rat IL-6 were performed. The affinity constant is 2.95 nM.

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Geprüfte Anwendungen

Produktinformation

83179-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test IL-6 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-6 fusion protein Eg0800
• Vollständiger Name: interleukin 6
• Berechnetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_012589.2
• Gene symbol: Il6
• Gene ID (NCBI): 24498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.