IL-6 Rekombinanter Antikörper

IL-6 Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240670A2

Kat-Nr. : 83747-9-PBS

Synonyme

IL6, B-cell stimulatory factor 2, IFN-beta-2, IL 6, Interferon beta-2

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  Sandwich ELISA standard curve of MP00687-4

  Sandwich ELISA standard curve of MP00687-4, Human IL6 Recombinant Matched Antibody Pair - PBS only. 83747-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0117. 83747-9-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

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  Sample test of MP00687-4

  Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL6.

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  Affinity and Kinetic Characterization of 83747-9-PBS

  Biolayer interferometry (BLl) kinetic assays of 83747-9-PBS against Human IL-6 were performed. The affinity constant is 0.468 nM.

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Geprüfte Anwendungen

Produktinformation

83747-9-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test IL-6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-6 fusion protein Eg0117
• Vollständiger Name: interleukin 6 (interferon, beta 2)
• Berechnetes Molekulargewicht: 212 aa, 24 kDa
• GenBank-Zugangsnummer: BC015511
• Gene symbol: IL6
• Gene ID (NCBI): 3569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.