IL-6 Monoklonaler Antikörper

IL-6 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

APC Fluorescent Dye

CloneNo.

4G3E6

Kat-Nr. : APC-69001

Synonyme

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, Interferon beta 2, Interleukin 6

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Galerie der Validierungsdaten

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  FC experiment of HUVEC using APC-69001

  1X10^6 HUVEC cells untreated (dashed lines) or treated with LPS and Brefeldin A (red) were intracellularly stained with 0.2 ug APC Anti-Human NeutraKine® IL-6 (APC-69001, Clone:4G3E6) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

• 

  FC experiment of HUVEC using APC-69001

  1X10^6 untrated or LPS and Brefeldin A treated HUVEC cells were intracellularly stained with 0.2 ug APC Anti-Human NeutraKine® IL-6 (APC-69001, Clone:4G3E6), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: mit LPS und Brefeldin A behandelte HUVEC-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

APC-69001 bindet in FC (Intra) IL-6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-6 fusion protein HZ-1019
• Vollständiger Name: interleukin 6 (interferon, beta 2)
• Gene symbol: IL6
• Gene ID (NCBI): 3569
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.