IL-7 Monoklonaler Antikörper

IL-7 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

1C11B11

Kat-Nr. : FITC-65281

Synonyme

IL7, IL-7, interleukin 7, Interleukin-7, 1C11B11, IL 7

---

• 

  FC experiment of human PBMCs using FITC-65281

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-7 (CL488-65281, Clone:1C11B11) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65281

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-7 (FITC-65281, Clone:1C11B11) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-7 (FITC-65281, Clone:1C11B11) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): LPS and Brefeldin A treated human PBMCs
• Erfolgreiche Detektion in FC: LPS and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65281 bindet in FC (Intra) IL-7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-7 fusion protein HZ-1281
• Vollständiger Name: interleukin 7
• Berechnetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC047698
• Gene symbol: IL-7
• Gene ID (NCBI): 3574
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)