IL-7Ra/CD127 Rekombinanter Antikörper

IL-7Ra/CD127 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240749H2

Kat-Nr. : 98074-1-RR

Synonyme

CD127, 240749H2, IL 7RA, IL 7R subunit alpha, IL 7R alpha

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  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3) and 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2), or Isotype Control. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2) (red), or Isotype Control(blue). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98074-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98074-1-RR against Human IL-7Ra/CD127 were performed. The affinity constant is 4.57 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98074-1-RR bindet in FC IL-7Ra/CD127 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: interleukin 7 receptor
• Berechnetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: AAC83204.1
• Gene symbol: CD127
• Gene ID (NCBI): 3575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).