IL-7Ra/CD127 Rekombinanter Antikörper

IL-7Ra/CD127 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242852B4

Kat-Nr. : 98385-1-PBS

Synonyme

CD127, Il7r, IL 7Ralpha, IL- 7Ralpha, IL-7 receptor subunit alpha

---

• 

  FC experiment of mouse splenocytes using 98385-1-RR (same clone as 98385-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 98385-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98385-1-RR (same clone as 98385-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed. This data was developed using the same antibody clone with 98385-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98385-1-RR (same clone as 98385-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug PE Anti-Mouse IL-7Ra/CD127 (A7R34) (PE-65093, Clone: A7R34). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 98385-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

98385-1-PBS bindet in FC IL-7Ra/CD127 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-7Ra/CD127 fusion protein Eg1826
• Vollständiger Name: interleukin 7 receptor
• Berechnetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_008372.4
• Gene symbol: CD127
• Gene ID (NCBI): 16197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).