IL-7Ra/CD127 Rekombinanter Antikörper

IL-7Ra/CD127 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242852B4

Kat-Nr. : 98385-1-RR

Synonyme

CD127, Il7r, IL 7Ralpha, IL- 7Ralpha, IL-7 receptor subunit alpha

---

• 

  FC experiment of mouse splenocytes using 98385-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98385-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98385-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug PE Anti-Mouse IL-7Ra/CD127 (A7R34) (PE-65093, Clone: A7R34). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98385-1-RR bindet in FC IL-7Ra/CD127 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-7Ra/CD127 fusion protein Eg1826
• Vollständiger Name: interleukin 7 receptor
• Berechnetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_008372.4
• Gene symbol: CD127
• Gene ID (NCBI): 16197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).