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- KD/KO Validated
IL-10 Monoklonaler Antikörper
IL-10 Monoklonal Antikörper für WB, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human und mehr (3)
Anwendung
WB, IHC, IF/ICC, ELISA, Cell treatment
Konjugation
Unkonjugiert
CloneNo.
8E4B6
Kat-Nr. : 60269-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | ag14870 |
Erfolgreiche Detektion in IF/ICC | MOLT-4-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 76 publications below |
IHC | See 41 publications below |
IF | See 25 publications below |
ELISA | See 2 publications below |
Produktinformation
60269-1-Ig bindet in WB, IHC, IF/ICC, ELISA, Cell treatment IL-10 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Kaninchen, Ratte, Zebrafisch |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | IL-10 fusion protein Ag14870 |
Vollständiger Name | interleukin 10 |
Berechnetes Molekulargewicht | 21 kDa |
Beobachtetes Molekulargewicht | 22 kDa |
GenBank-Zugangsnummer | BC104252 |
Gene symbol | IL-10 |
Gene ID (NCBI) | 3586 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for IL-10 antibody 60269-1-Ig | Protokoll herunterladen |
IHC protocol for IL-10 antibody 60269-1-Ig | Protokoll herunterladenl |
IF protocol for IL-10 antibody 60269-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Bioact Mater A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds | ||
Adv Healthc Mater One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing. | ||
J Control Release 3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing | ||
J Immunother Cancer Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma. | ||
Mol Ther Host liver-derived extracellular vesicles deliver miR-142a-3p induces neutrophil extracellular traps via targeting WASL to block the development of Schistosoma japonicum. |