• Featured Product
  • KD/KO Validated

IL-10 Monoklonaler Antikörper

IL-10 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(137)

CloneNo.

8E4B6

Kat-Nr. : 60269-1-Ig

Synonyme

IL-10,IL10, 8E4B6, CSIF, IL 10, IL10



Geprüfte Anwendungen

Erfolgreiche Detektion in WBag14870
Erfolgreiche Detektion in IF/ICCMOLT-4-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:8000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60269-1-Ig bindet in WB, IHC, IF/ICC, ELISA, Cell treatment IL-10 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Kaninchen, Ratte, Zebrafisch
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen IL-10 fusion protein Ag14870
Vollständiger Name interleukin 10
Berechnetes Molekulargewicht 21 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC104252
Gene symbol IL-10
Gene ID (NCBI) 3586
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-10 antibody 60269-1-IgProtokoll herunterladen
IHC protocol for IL-10 antibody 60269-1-IgProtokoll herunterladenl
IF protocol for IL-10 antibody 60269-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IHC

Bioact Mater

A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds

Authors - Shunlai Shang
WB

Adv Healthc Mater

One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing.

Authors - Shen Guo
rabbitIF

J Control Release

3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing

Authors - Yuehe Xu
WB

Mater Today Bio

Injectable multifunctional CMC/HA-DA hydrogel for repairing skin injury.

Authors - Longlong Cui
humanIHC

J Immunother Cancer

Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma.

Authors - Yuwei Deng
WB

Mol Ther

Host liver-derived extracellular vesicles deliver miR-142a-3p induces neutrophil extracellular traps via targeting WASL to block the development of Schistosoma japonicum.

Authors - Lifu Wang