IL-11RA Polyklonaler Antikörper

IL-11RA Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10264-1-AP

Synonyme

IL 11 receptor subunit alpha, IL 11R alpha, IL 11R subunit alpha, IL 11RA, IL11RA, IL-11RA, interleukin 11 receptor, alpha

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Galerie der Validierungsdaten

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  WB analysis of mouse colon using 10264-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10264-1-AP (IL11RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10264-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10264-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 10264-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 10264-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of N2a cells using 10264-1-AP

  IF result of anti-IL11RA (10264-1-AP, 1:200) with N2a cells (4%PFA fixed) by Miss Masue Marbiah, PhD student MRC Prion Unit, Department of Neurodegenerative Diseases, UCL Institute of Neurology.

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  FC experiment of K-562 using 10264-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human IL-11RA (10264-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: N2a-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

10264-1-AP bindet in WB, IHC, IF, FC, ELISA IL-11RA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-11RA fusion protein Ag0373
• Vollständiger Name: interleukin 11 receptor, alpha
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC003110
• Gene symbol: IL11RA
• Gene ID (NCBI): 3590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-11 (IL-11), a member of the IL-6 family of cytokines, was firstly identified in bone marrow-derived stromal cells (PMID: 2145578). IL-11 is a multifunctional cytokine that plays important roles in hematopoiesis, bone development, tissue repair, and tumor development (PMID: 33863879). IL-10 signals through a receptor complex consisting of IL-11 receptor alpha subunit (IL-11RA) and gp130. IL-11RA binds IL-11 and gp130 transmits signals to the nucleus via Janus kinase (JAK) activation. In addition to a membrane-bound form, IL-11RA can also exist as a soluble form (sIL-11RA) that acts as an agonist of IL-11 activity (PMID: 26876177; 27493449).

Publikationen

Species	Application	Title
mouse	IF	EMBO J; Identification of a gene regulatory network associated with prion replication.; Authors - Masue M Marbiah
mouse	WB	ACS Nano; Nanoparticle-Mediated RNA Therapy Attenuates Nonalcoholic Steatohepatitis and Related Fibrosis by Targeting Activated Hepatic Stellate Cells; Authors - Chenshuang Zhang