IL-12A Monoklonaler Antikörper

IL-12A Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

6A10A8

Kat-Nr. : 60324-1-Ig

Synonyme

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12A, IL-12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 60324-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60324-1-Ig (IL12A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 60324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 60324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60324-1-Ig bindet in WB, IHC,ELISA IL-12A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-12A fusion protein Ag17625
• Vollständiger Name: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC104982
• Gene symbol: IL12A
• Gene ID (NCBI): 3592
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.